MEDICINE AND SURGERY "F"
Course of LABORATORY MEDICINE
Disturbances of the endocrine system

HORMONES

      Hormones are produced by endocrine glands and serve to regulate the physiological functions of many organs and tissues. Their concentration in the blood is usually finely regulated thus their concentration and its circadiane changes vary within a limited range. Disregulation may occur both in the sense of excess secretion (often due to benign, hormone-secreting tumours of the gland) or in the sense of insufficient secretion (possibly due to damage of the gland due to metabolic defects, malignancies, insufficient stimulation). Moreover, alterations of the circadian rythms of secretion occur and in some instances cause symptoms and have diagnostic value.
      From a biochemical standpoint, hormones belong to several different families of compounds, and every endocrine gland may secrete one or more hormones, usually belonging to the same biochemical family. An exception is the thyroid which secretes two hormones belonging to different biochemical families: Thyroxin is an aminoacid derivative, whereas Thyrocalcitonin is a small protein. The adrenals do secrete hormones of two very different chemical types (catecholamines are aminocid derivatives, corticoids are cholesterol derivatives), but form an embriological viewpoint they are two different glands one of which encircles the other. As a consequence of this variety several analytical methods are required, as summarized in the Table below:

HORMONES AND THE CLINICAL BIOCHEMISTRY LABORATORY
Endocrine glandHormoneChemical natureMeasurement method
Epiphysis (pineal gland)MelatoninTyrosine derivativeExtraction in dichloromethane followed by HPL Chromatography
 
Neurohypophysis (posterior pituitary) Vasopressin (Anti Diuretic Hormone, ADH) Polypeptide Immunoassay
Oxytocin Polypeptide Immunoassay; mass spectrometry
 
Adenohypophysis (anterior pituitary) Growth Hormone (GH) Protein Immunoassay (biological test)
Gonadotropins (LH and FSH) Proteins Immunoassay (biological test)
Thryoid stimulating hormone (TSH) Protein Immunoassay
Adrenal Cortex stimulating hormone (ACTH) Protein Immunoassay
 
ThyroidThyroxine (tetraiodothyronine) and triiodothyronine (T4 and T3)Tyrosine derivativeRadioImmunoAssay
Thyrocalcitonin Protein Immunoassay
 
PancreasInsulinProteinImmunoassay
Glucagon Protein (Immunoassay)
 
Adrenal cortexGlucocorticoid hormones (e.g. cortisol)Cholesterol derivativesRadioImmunoAssay, usually after extraction with organic solvents
Mineralocorticoid hormones (e.g. aldosterone) Cholesterol derivatives RadioImmunoAssay, usually after extraction with organic solvents; chemiluminescence
Sexual hormones (e.g. estrogens, progesterone, testosterone) Cholesterol derivatives RadioImmunoAssay, usually after extraction with organic solvents
 
Adrenal medullacatecholamine hormones (e.g. adrenaline)Tyrosine derivatives HPL Chromatography
 
Measurements methods in parentheses are possible, but not commonly used.

      Disorders of the anterior pituitary.
      Hypopituitarism can be selective or generalized (panhipopituitarism). Generalized hypoituitarism is the result of compressive or ischemic destruction of the hypophysis, often due to tumours; a less common condition is the Sheehan syndrome (ischemic necrosis of the hypophysis due to post partum hemorrages and shock). Gonadotropins are lost first, followed by GH, and then by TSH and ACTH. Death may ensue, due to the lack of thyroid and adrenal stimulation. Diagnosis is by imaging methods (lesions of the sella turcica) and by laboratory determination of the hormones' concentration in the serum.
      Selective hypopituitarism, with isolated pituitary deficiencies, is suspected because of failure to grow (pituitary nanism due to deficiency of GH), or because of hypothyrodism or adrenal insufficiency (Addison's disease), or sterility and lack of menstruations.
      Hyperpituitarism, i.e. the pathological increase of pituitary function, is usually selective and may be idiopathic or due to a benign neoplasia of the gland that secretes one of its hormones. In the case of GH the syndrome causes pituitary gigantism (in the child) or acromegaly (in the adult). Increased secretion of ACTH leads to the pituitary form of the Cushing disease, and the symptoms are due to the increased production of cortisol.

      Disorders of the posterior pituitary.
      Diabetes insipidus is due to insufficient or absent production of ADH. Since this hormone causes the kidney to reabsorb water, its deficiency is associated with poliuria and diluted urine (to be distinguished from diabetic mellituria, in which the urine flow is increased because of the osmotic effect of glucose, and from nephrogenic diabetes insipidus, in which the kidney's function is reduced).

      Disorders of the thyroid.
      Hypothyroidism may be due to lack of iodine in the diet, or to inflammatory diseases of the gland. The thyroid is usually enlarged (goiter) and the concentration of T4 and T3 in the blood is decreased. The clinical picture is ususally quite characteristic and diagnosis is confirmed by laboratory findings. If iodine administration does not cure the syndrome a substitutive therapy is started.
      Hyperthyroidism (Basedow's disease; Graves' disease; thyrotoxicosis) may be due to diffuse enlargement of the gland (in some causes because of a benign adenoma of the pituitary, which secretes TSH) or to a secreting adenoma. 131I scintigraphy is diagnostic, and the laboratory analyses will confirm increased serum concentration of T3 and T4 (and possibly of TSH). Surgery is indicated.

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